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For many years freezing semen samples have been a possibility.Human semen was first successfully cryopreserved, with subsequent pregnancies and births in 1953.

Nowadays freezing semen and testicular tissue samples are a routine procedure in the embryological laboratory. The samples can be frozen before a treatment cycle to be thawed when needed.

Equally important is the cryopreservation of embryos. If a sufficient number embryos are available, then one option is to freeze the surplus (spare) embryos for possible use at a future date.

Usually embryos are frozen on the first day after fertilization, on the second day (4 cell stage) or at the stage of blastocyst formation (5/6 days after fertilization).

Cryopreservation gives us the possibility to store the embryos that will not be transferred in the present cycle for a later date without the cost and inconvenience of a stimulated IVF cycle. Also, in rare occasions when we wish to delay the transfer to avoid the risk of ovarian hyperstimulation syndrome or when we wish to wait for a time when endometrial receptivity will give us a better chance of pregnancy.

 

Storage is in liquid nitrogen at –196*C.

Although there is no theoretical time limit of storage you will be asked to sign a consent form according to the IVF center policy / IVF legislation ascertaining to the length of time allowed for Cryopreservation.

If the couple at a future date wishes to transfer their embryos/semen to another IVF center this is of course a possibility by transporting their samples in miniature liquid nitrogen containers can “Dry Shippers”.

read more about the technique Vitrification

  • Fertilized oocyte

    The fertilized oocyte has 2 pro-nuclei (one containing the chromosomes from the male –carried by the sperm...

  • Spermatozoa

    How spermatozoa is prepared for IUI & IVF Density gradient centrifugation ( formally known as the percoll gradient )

  • Human Oocyte

    All eggs are not the same! Have you heared your doc say "immature eggs", "poor eggs" etc?

  • Four Cell Embryo

    How embryos are usually graded is by morphological observation...

  • Eight Cell Embryo

    The embryologist observes the development in vitro from a single cell unitil the embryo-transfer...

  • Blastocyst

    Blastocyst develop 5 or 6 days after fertilization...

  • Hatching Blastocyst

    Before implantation the blastocyst has to escape its outer coat

  • Apoptosis of Sperm cells
  • Technique IMSI

    Pre-selection of spermatozoa for ICSI with higher magnif....

  • Technique IMSI 2
  • Micromanipulation
  • Time Lapse Monitoring Of Embryos

    Remote monitoring embryos avoids the practice of removing them from their incubation...

  • Karyotype

    The study of your chromosomes

  • IVF Lab

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