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Giles ivf

Videos

Videos

Click on thumbnails to watch the videos-
News items, IVF techniques and some videos
"just for fun"



Preparation. Early blastocyst. Metabolomics 1. Metabolomics 2.
hatching blastocyst metabolomix metabolomics
Culture medium 
prepared
for the following
day's
egg collection.
Personal archives-
Giles Palmer/
T.Triantafillou.
Metabolomics-how to
use
this technology
.
Αnimated guide of
this
new techology.
Helping the
embryologist choose
the
best embryo!
       

   line1

Embryo lab Embryo transfer. Insemination-IVF.  Oocyte.
lab embryolab fert fertilized
The embryos are
housed throughout
their stay in the
laboratory in
special incubators.
The fertilized oocytes
are cultured until the
day of embryo
transfer.
100,000 spermatozoa
are added to each
egg and incubated
until the next morning.

The fertilised oocyte,
showing two
pronuclei.
One from
each parent.

       

 

Embryo freezing. Micro-injection1. Micro-injection2. Metabolomics.
freeze icsi icsi weeeeeeeeeeeeee
Since 1984, thousands of
babies have been born
worldwide after having
undergone embryo
cryopreservation.
The sperm must be
immobilized before
injecting it into the oocyte.
Sperm is placed inside
the oocyte with a special
pipette.
Alter news report about
promising new technique
to reduce multiple
pregnancies and increase
IVF success.
       

gg 

Egg pick-up. Sperm motility 1. Sperm motility 2. Lab cam.
prep kill sperm sperms
Oocytes are collected and
placed in culture media,
incubated at 37*C under
special conditions until
insemination.
Spermatozoa in ICSI
medium to slow down their
motility, enabling the
embryologist to select the
best sperm for ICSI.
Sperm exhibiting increased
motility after purification.
"inside look" view of the
in vitro fertization
embryology lab
       

gg 

Sperm on zona Sperm Prep Testicular Sperm TESE Sperm
zona ang tese teze
Single sperm attempting
to penetrate an oocyte,
burrowing into the outer
coating.
The semen sample is
accessed, centrifused,
counted & supplemented
with culture media.
In cases of azospermia-
a testicular biopsy can tell
us if there spermato-
genesis
Choosing spermatozoa
from testiclular
tissue.
       

nn 

Fertilisation check. The first day. Development. Evaluation 2
pronuclei spare x me
Fertilization is confirmed by
observing 2 pro-nuclei.
The embryologist observes
the oocytes and checks for
signs of fertilization.
Embryo development  from
fertilization to blastocyst.
Two pro nulcei are clearly
seen..signs of fertilization
       

hh 

Embryo biopsy. Blastocyst. Hatching Embryo Freezing.
biopsy blastocyst hatching freeze
A cell is extracted from the
8 cells-embryo. The cell is
then used for genetic
analysis.
A fertilized egg after 5 days
becomes a blastocyst-
an embryo ready to implant
A hole tiny hole is made in
the outer protein coat
surrounding the embryo.
Cryopreservation gives us
the possibility to store the
embryos that will not be
transferred.
       

 jj

Micro manipulation. Embryologists. kosmofert1. kosmofert2.
manipulation egg human fertilization metabolomics1
Using a powerful micro-

scope & micromanipulator
-the egg is controlled by
delicate joysticks

The reproductive biologists
at work in the lab.
Fertilization lab.
Fertilization in a dish!
       

 

hh

Time lapse videos Time lapse videos Time lapse videos Time lapse videos
development tube crios metabolomics1
Cryo-Innovation's new tool
records early embryo
development.
All the embryos of one
patient from the 2PN
stage untill the 1st and
2nd cleavage.
The first modular
timelapse microscopy
system.
1 cell stage till hatching
From  PRIMO Vision (Cryo-Innovation Ltd. Budapest, Hungary)

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