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F.I.S.H.

 Fotolia DNA

FISH (Fluorescent in-situ hybridization)

The application of multi-coloured FISH has been used for the analysis of both gender determination (a.k.a. sexing), for X-linked recessive disorders (e.g. haemophilia), aneuploidy screening (e.g. Abnormal number of chromosomes) and translocations (e.g. Robertsonian).
X-linked recessive diseases are caused by genetic defects on the X-chromosome and are usually transmitted by women carriers with a 50% risk of having male offspring hemizygous for the condition, hence affected. Therefore by sexing the embryos and transferring only those which are female, there is a 50% of transferring a normal female and a 50% chance of transferring a carrier that will not be affected by the disease.

Ivf fish Fish_probes


Chromosome specific colored probes

Multicoloured FISH analysis using probes for X, Y, 18 and 13/21 show that approximately 40% of the oocytes of women over 40 years of age test positive for aneuploidy. There are now recently developed 5-colour FISH chromosomal probes that allow testing for 5 different chromosomes in 4-5 hours.

 

  • Fertilized oocyte

    The fertilized oocyte has 2 pro-nuclei (one containing the chromosomes from the male –carried by the sperm...

  • Spermatozoa

    How spermatozoa is prepared for IUI & IVF Density gradient centrifugation ( formally known as the percoll gradient )

  • Human Oocyte

    All eggs are not the same! Have you heared your doc say "immature eggs", "poor eggs" etc?

  • Four Cell Embryo

    How embryos are usually graded is by morphological observation...

  • Eight Cell Embryo

    The embryologist observes the development in vitro from a single cell unitil the embryo-transfer...

  • Blastocyst

    Blastocyst develop 5 or 6 days after fertilization...

  • Hatching Blastocyst

    Before implantation the blastocyst has to escape its outer coat

  • Apoptosis of Sperm cells
  • Technique IMSI

    Pre-selection of spermatozoa for ICSI with higher magnif....

  • Technique IMSI 2
  • Micromanipulation
  • Time Lapse Monitoring Of Embryos

    Remote monitoring embryos avoids the practice of removing them from their incubation...

  • Karyotype

    The study of your chromosomes

  • IVF Lab

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