Monday, May 27th

Last updateSun, 21 Feb 2021 9am

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G Nikas2




Inside an in vitro fertilisation
Laboratory / Quality Control

About the author

You tube videos-techniques explained

I realise that the laboratory where we work is often a place of great mystery and intrigue to you. For us it’s all very straightforward. We spend most of my working time here, after all. But you don’t get the opportunity to see what happens between the collection of your sperm and eggs and your embryo transfer.

What’s going on in those stressful times when nothing seems to be happening in their treatment. Why do they have to wait? And what exactly are we doing to their precious eggs, sperms and growing embryos?

So here’s a chance for you to see your treatment cycle through the eyes of your embryologists on you tube







 1 Incubator
2.Joy-stick for ICSI
3.UPS Power (continious power supply)
4. Monitor for observation of ICSI
5.Micro-tool holder on Inverted microscope
6.Warming stage/7.Icsi Dish/8.Joy-stick for ICSI
9.Coarse manipulation joystick
10. Syringe controlling the manipulation of gametes
11.Corian Walls. Non porous, anti-bacterial
12.Anti-vibration table
13.Central cables enclosed in s. steel band
14.Microscope link to computer
15. Anti-bacterial, anti-static floor
16.Anti-vibration, cushioned legs of table
ICSI Station





1.Laminar flow cabinet: clean air for work surfaces
2.Control unit for laminar flow cabinet.
3.Air-tight window-access to operating theatre
4.Control unit for environmental conditions in lab
5.Stereo microscope
6.Plexi-glass protection shield
7.Incubator (close to work area)
8.Central cables enclosed in s. steel band
9.Microscope link to computer
10.Special gas inlet control unit
11.Inverted Microscope
12.Working surface at 37*C
13.Pipette gun for dispensing media
14.Non-toxic pencils for use in lab/15.Chair

Work Station









1.HEPA (High Efficiency Particulate Air) Filters
2.Anti-bacterial sealed panels
3.Filter integrity monitor
4.Positive pressure monitor
5.Corian walls
6.Sealed light fittings

Air lock and air filtration with Hepa filters













1.Liquid nitrogen embryo storage bank
2.Temperature sensor linked to alarm system
3.Easy mobility by roller wheels
4.Stainless steel floor to assure cleanliness

Liquid nitrogen Tanks




1.Special gas bottles supplying the incubators have fail-safe system to
ensure constant supply.
A separate gas line (stainless steel) for each incubator.

Gas Supply System



Quality Control in the Lab.


ivf In a Clean-room environment, positive pressure must be preserved. Ultra clean air first
enters the lab. and is pushed out to less critical areas. Here, we can see the testing of the
pressure difference between two rooms.
ivf As above. Note air tight seal between lab. and operating theatre.
ivf Harmful chemicals in the air can be detected by use of VOC badges. Especially important
when constructing a new lab or addition of new equipment into the lab.
ivf We don't just rely on the temperature reading given by the incubators or work surfaces-
daily calibration is monitored.
ivf As above. Temperature measurement of the warm surface where we work.
ivf The pH of the culture media which nurishes the growing embryo is very important!!
We check for the correct pH from inside the incubator!
ivf Thermo coins help us check the work surface temperature.
ivf All work surfaces can be adjusted for the correct temperature.
ivf Security is a must!
All critical rooms and laboratories have restricted access and a PIN is required.
ivf The efficiency of the filters in the work cabinates are tested.
ivf A particle counter reports on the quality of the air.
see ACC-Kalavrias website
ivf Again, filter efficiency is monitored.
ivf Special gas mixture that helps the embryo develop is quality assured
enters the lab through stainless steel pipes-but we add an extra filter
before entering the incubator.
ivf To enter the lab each embryologist has to pass through an air lock/air shower, seen in
the background. Note the embryologist does not wear the normal hospiatl "greens".
Special clothing, no pockets, high neck and long sleeved help reduce contamination.
The material emits no fibres into the environment, and is specially laundered.