Monday, May 27th

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Oocyte Morphology

nice nice nice nice

 

All eggs are not the same!

Have you heared your doc say "immature eggs", "poor eggs" etc?

Well, it's not unusual for some of the oocytes collected at the egg pick up to be unsuitable for fertilization ( and remember not all your eggs fertilized). Think twice if ever asked to donate eggs to another couple! link

Superovulation aims to produce a certain number of nice eggs-the time of egg collection is important to harvest a suitable number of eggs. This is determined my size of follicles (on an ultrasound) and hormone levels (eostrodiol blood test).

A nice egg or oocyte should have:

Mature outer cells to allows sperm to weave their way towards the egg.
Mature outer jelly coat to allow the entry of only one sperm.
Mature cell at the correct stage of development containing half the genetic material (to fuse with the sperm containing the other half) giving rise to an embryo ( a mix a both parents)

 
Anatomy of an egg:

egg cytoplasm

the cytoplasm- the main mass of the egg, the cellular material,
DNA and energy store are housed here

polar egg

The polar body- indicates the egg is at the correct stage for
fertilization ( contains the excess DNA material)

egg cells-embryo

  
The cummulus cells-surround the egg after ovulation and 
gradually are dispersed

zonae egg

The zona pellucida- jelly like coating of the egg and developing 
embryo

 

 

Immature eggs

egg-picture immature

The egg with no polar body

gv immature-egg

 The germinal vesical-indicates the egg is very immature and 
will not fertilize

germinal germ

 The germinal vesical- has not completed cell division to contain
half the genetic material

 

germinal egg

The germinal vesical-the inner circle containing the chromosomes

germinal

 

Abnormal eggs

egg21  

 The egg with abnormal shape

The egg with vacuoles -fluid sacs for discarding cellular matter

 

The egg with abnormal cytoplasm

 

egg28  

The egg with abnormal polar body

 

egg26  

The egg with no zona

 

egg22  

Empty zona-only zona pellucida remains after egg degenerates

 

 

  • Fertilized oocyte

    The fertilized oocyte has 2 pro-nuclei (one containing the chromosomes from the male –carried by the sperm...

  • Spermatozoa

    How spermatozoa is prepared for IUI & IVF Density gradient centrifugation ( formally known as the percoll gradient )

  • Human Oocyte

    All eggs are not the same! Have you heared your doc say "immature eggs", "poor eggs" etc?

  • Four Cell Embryo

    How embryos are usually graded is by morphological observation...

  • Eight Cell Embryo

    The embryologist observes the development in vitro from a single cell unitil the embryo-transfer...

  • Blastocyst

    Blastocyst develop 5 or 6 days after fertilization...

  • Hatching Blastocyst

    Before implantation the blastocyst has to escape its outer coat

  • Apoptosis of Sperm cells
  • Technique IMSI

    Pre-selection of spermatozoa for ICSI with higher magnif....

  • Technique IMSI 2
  • Micromanipulation
  • Time Lapse Monitoring Of Embryos

    Remote monitoring embryos avoids the practice of removing them from their incubation...

  • Karyotype

    The study of your chromosomes

  • IVF Lab

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