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In vitro Fertilisation

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News items, IVF techniques and some videos
"just for fun"

Day -1:Preparation.	Early blastocyst.	Metabolomics 1.	Metabolomics 2.

Culture medium prepared for the following day's egg collection.	Personal archives- Giles Palmer/ T.Triantafillou.	Metabolomics-how to use this technology.	Αnimated guide of this new techology.Helping the embryologist choose the best embryo!

Embryo lab Incubator.	Day 2. Embryo transfer.	Day 0. Insemination-IVF.	The fertilised oocyte.

The embryos are housed throughout their stay in the laboratory in special incubators.	The fertilized oocytes are cultured until the day of embryo transfer.	100,000 spermatozoa are added to each egg and incubated until the next morning.	The fertilised oocyte, showing two pronuclei. One from each parent.

Steps in embryo freezing.	Micro-injection1.	Micro-injection2.	Metabolomics@mitera.

Since 1984, thousands of babies have been born worldwide after having undergone embryo cryopreservation.	The sperm must be immobilized before injecting it into the oocyte.	Sperm is placed inside the oocyte with a special pipette.	Alter news report about promising new technique to reduce multiple pregnancies and increase IVF success.

Day 0. Egg pick-up.	Sperm motility 1.	Sperm motility 2.	Lab cam.

Oocytes are collected and placed in culture media, incubated at 37*C under special conditions until insemination.	Spermatozoa in ICSI medium to slow down their motility, enabling the embryologist to select the best sperm for ICSI.	Sperm exhibiting increased motility after purification.	"inside look" view of the in vitro fertization embryology lab

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sperm on zona	Day0: Sperm Prep	TESE : Testicular Sperm	TESE motile sperm

Single sperm attempting to penetrate an oocyte, burrowing into the outer coating.	The semen sample is accessed, centrifused, counted & supplemented with culture media.	In cases of azospermia- a testicular biopsy can tell us if there spermato- genesis	Choosing spermatozoa from testiclular tissue.

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Day 1: Fertilisation check.	The first day evaluation.	Embryo development.	First day evualtion 2

Fertilization is confirmed by observing 2 pro-nuclei.	The embryologist observes the oocytes and checks for signs of fertilization.	Embryo development from fertilization to blastocyst.	Two pro nulcei are clearly seen..signs of fertilization

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Embryo biopsy Athens.	Blastocyst.	Helping embryo to hatch.	Day2(Embryo Freezing).

A cell is extracted from the 8 cells-embryo. The cell is then used for genetic analysis.	A fertilized egg after 5 days becomes a blastocyst- an embryo ready to implant	A hole tiny hole is made in the outer protein coat surrounding the embryo.	Cryopreservation gives us the possibility to store the embryos that will not be transferred.

Day X (Micro manipulation).	Embryologists.	kosmofert1.	kosmofert2.

Using a powerful micro- scope & micromanipulator -the egg is controlled by delicate joysticks	The reproductive biologists at work in the lab.	Fertilization lab.	Fertilization in a dish!

Time lapse videos	Time lapse videos	Time lapse videos	Time lapse videos

Cryo-Innovation's new tool records early embryo development.	All the embryos of one patient from the 2PN stage untill the 1st and 2nd cleavage.	The first modular timelapse microscopy system.	1 cell stage till hatching
From PRIMO Vision (Cryo-Innovation Ltd. Budapest, Hungary)

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